Rhodococcus opacus DSM 1069 utilized pine organosolv pretreatment effluent as a sole carbon and energy source for 120hat 1.5 w/v% solids concentration and accumulated a maximum of 26.99±2.88% of its cellular dry weight in oils composed of oleic, palmitic, and stearic fatty acids. These results establish the potential for lignocellulosic pretreatment effluent as a feedstock for microbial biodiesel production via oleaginous R. opacus and an interesting route for biorefinery waste stream optimization.
Wells, T., Wei, Z., & Ragauskas, A. (2015). Bioconversion of lignocellulosic pretreatment effluent via oleaginous Rhodococcus opacus DSM 1069. Biomass and Bioenergy, 72, 200–205. https://doi.org/10.1016/j.biombioe.2014.11.004