Transcription factor Lbx1 expression in mouse embryonic stem cell-derived phenotypes

6Citations
Citations of this article
14Readers
Mendeley users who have this article in their library.

Abstract

Transcription factor Lbx1 is known to play a role in the migration of muscle progenitor cells in limb buds and also in neuronal determination processes. In addition, involvement of Lbx1 in cardiac neural crest-related cardiogenesis was postulated. Here, we used mouse embryonic stem (ES) cells which have the capacity to develop into cells of all three primary germ layers. During in vitro differentiation, ES cells recapitulate cellular developmental processes and gene expression patterns of early embryogenesis. Transcript analysis revealed a significant upregulation of Lbx1 at the progenitor cell stage. Immunofluorescence staining confirmed the expression of Lbx1 in skeletal muscle cell progenitors and GABAergic neurons. To verify the presence of Lbx1 in cardiac cells, triple immunocytochemistry of ES cell-derived cardiomyocytes and a quantification assay were performed at different developmental stages. Colabeling of Lbx1 and cardiac specific markers troponin T, -actinin, GATA4, and Nkx2.5 suggested a potential role in early myocardial development. © 2011 Stefanie Schmitteckert et al.

Cite

CITATION STYLE

APA

Schmitteckert, S., Ziegler, C., Kartes, L., & Rolletschek, A. (2011). Transcription factor Lbx1 expression in mouse embryonic stem cell-derived phenotypes. Stem Cells International. https://doi.org/10.4061/2011/130970

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free