Purification and characterization of the CelB endoglucanase from Streptomyces lividans 66 and DNA sequence of the encoding gene

Abstract

The endoglucanase CelB isolated from culture filtrates of Streptomyces lividans IAF9 has an M(r) of 36,000. With carboxymethyl cellulose as the substrate, the V(max) and K(m) values are 110 IU/mg of enzyme and 1.3 mg/ml, respectively. Comparison of primary amino acid sequences classifies CelB in the H family of cellulases.