Comparison of the histological methods in the diagnostic of deer cysticercosis

Abstract

Histochemical methods for the detection and diagnosis of the developmental stages of the canine tapeworm, from the genus Taenia found in the heart and lungs of red deer (Cervus elaphus) and roe deer (Capreolus capreolus) hunted in Eastern Slovakia, is presented here. Detailed morphology of cysticerci (Cysticercus spp.), based on microscopic and histochemical analysis is described. For confirmation and demonstration of PAS-positive substances in the body of parasitic tissue (tegument and mesenchyme) the McManus - PAS method was used. The histochemical method according to Van Kossa was very effective for confirmation of calcareous corpuscles, which are one of the most important histological markers of cestode tissues (larva or adult). © 2008 Versita and Springer-Verlag.