Protective Effects of Grape Molasses and Resveratrol Against DMBA Induced Oxidative Stress in Rat Ovarian Tissues

Abstract

Objective: The polyphenolic compound, resveratrol (3, 4’, 5-trihydroxystilbene), trans-resveratrol is a natural phytoalexin that exists in many different foods such as grape peel and seed, peanut, mulberry and blueberry. Molasses is a natural food obtained by boiling and concentrating fruit juices known to be rich in minerals such as iron, phosphorus, potassium, sodium, calcium and magnesium, and phosphoric acid, formic acid, organic acids and some vitamins. Polyphenolic compounds are natural antioxidants and are known to have protective effects against tissue damage caused by reactive oxygen species (ROS). In this study, we aimed to investigate the protective effects of grape molasses and resveratrol on ovarian damage induced by 7, 12-dimethylbenz [a] anthracene (DMBA). Methods: A total of 42 old female Wistar Albino rats, aged 18 weeks were divided into six groups. 10 mg/kg DMBA was injected in the rats in DMBA group subcutaneously on day 0 and day 7 while grape molasses feed with 20% grape molasses was given along with DMBA application to the rats in DMBA + grape molasses group. Resveratrol was administered by subcutaneous injection at 10 mg/kg/day to the DMBA + Resveratrol group, while only feed with 20% grape molasses was given to the grape molasses group. Resveratrol was administered by subcutaneous injection at 10 mg/kg/day to the resveratrol group. Results: GSH activity between the control group and molasses group, DMBA and DMBA + molasses groups and the control group and the DMBA + grape molasses groups was found statistically significant. Regarding the NO activity, the difference between the DMBA and resveratrol groups, DMBA and DMBA + resveratrol groups and the grape molasses and resveratrol groups was found statistically significant. MDA activity between DMBA + resveratrol and DMBA + grape molasses groups, and DMBA + grape molasses and grape molasses groups was found statistically significant. Conclusion: Molasses as one of the most important nutrient sources of Eastern and Central Anatolia was observed to decrease ovarian tissue oxidative damage induced with DMBA compared to resveratrol.