Development of the moss Pogonatum urnigerum (Hedw.) P. Beauv. under in vitro culture conditions

Abstract

Pogonatum urnigerum (Polytrichaceae) in vitro culture was established from spores collected in nature. Both protonema and gametophore stages of gametophyte development were obtained. Also, a stable callus culture was established using hormone-free nutrient medium. The best nutrient medium for development was half-strength Murashige- Skoog medium supplemented with 1.5% sucrose. Auxin treatment enabled some gametophores to develop, but prolonged treatment induced early senescence. Tissues grown on cytokinin did not produce any gametophytes and did not survive prolonged treatment.Sterilna kultura mahovine Pogonatum urnigerum (Polytrichaceae) uspostavljena je iz spora sakupljenih u prirodi. Gajenjem na hranljivoj podlozi bez biljnih hormona iz spora su se razvile protoneme i gametofiti. Uspostavljena je i stabilna kultura kalusa. Ustanovljeno je da je za gajenje ove vrste najbolja hranljiva podloga koja sadrzi smanjenu koncentraciju soli i vitamina po Murashige-Skoog-u i 1.5% saharozu. Tretman auksinom nije pospesio razvice gametofita, dok je produzeni tretman doveo do postepenog odumiranja protoneme. Tkiva gajena na podlozi koja je sadrzala citokinine uopste nisu produkovala gametofite i nisu prezivela produzeni tretman ovim hormonom. Ovaj rad predstavlja prvu studiju razvica vrste Pogonatum urnigerum u in vitro kulturi.