Micro-quantity tissue digestion for metal measurements by use of a microwave acid-digestion bomb

Abstract

We describe a simple and convenient method for processing small amounts of tissue samples for trace-metal measurements by atomic absoprtion spectrometry, by use of a modified Parr microwave digestion bomb. Digestion proceeds rapidly (≤ 90 s) in a sealed Teflon-lined vessel that eliminates contamination or loss from volatilization. Small quantities of tissue (5-100 mg dry weight) are digested in high-purity nitric acid, yielding concentrations of analyte that can be measured directly without further sample manipulation. We analyzed National Institute of Standards and Technology bovine liver Standard Reference Material to verify the accuracy of the technique. We assessed the applicability of the technique to analysis for aluminium in bone by comparison with a dry ashing procedure.