Preparation of chiamydomonas chloroplasts for the in vitro import of polypeptide precursors

Abstract

To study the import of polypeptide precursors we have adapted and compared two procedures for the isolation of competent chloroplasts from the green unicellular alga, Chlamydomonas reinhardtii: silicasol gradient centrifugation and elutriation. The chloroplasts actively import the precursor of the small subunit of ribulose bisphosphate carboxylase-oxygenase in vitro. © 1989 American Society of Plant Biologists. All rights reserved.