Pengaruh Berbagai Perlakuan Fisik Terhadap Molekul dan Aktivitas Protein Antibakteri dalam Cairan Selom Cacing Tanah Perionyx Excavatus

Abstract

Abstreet Eqtlworms oe itwertebrde with tle wnq& immtme rystem, since they we able to tnld out brcterium pathogen in Srou,ing medfum Their immtme system consists of a lat of reactiotu inchding: fagwytosis, encapsuldion, aglutindion, opsonisation ana rys*76, foreign component attackcd There te some small molecales ond extra cellular proteins dilue in coelomic etthv'orm that play ot important role in immwte sysiem od rylibrcterial agent. Tlw presmt expertment was done to study the ffict'of several dfferent tredments (feeding living, mediun electricity stimtlaion) ii ttrc molecales resistance od activity of anti brcterial protein in coelomic of earthworms. Coelomic diluion was extrrcted by using electricily stimulation at 6 voli, and the rcttvtty of anti bacterial protein was meastred by spectrophotometer, and agr diffusion. Proiein extra eelldt moleculs size in coelomic dil*ion was observed by Sodhm Daodecil Sulphae (SDS) electroplnresis. Results showed tM tte activtty of anti bacterial protiin n coelomic dilution, md molecule with 29 kDa size were deleied by tredments.-Extraction of coelomic dilution which contained of rctivity of otti bacteriol protein could be done in feces from cow which was never stimulated before.