The effect of encapsulated nitrate and monensin on ruminal fermentation using a semi-continuous culture system

Abstract

Because enteric methane (CH4) production from ruminants represents a source of greenhouse gas emissions and an energy loss for the host animal alternatives to minimize emissions is a current research priority. Seven 37-d trials tested the effect of encapsulated nitrate (EN) and sodium monensin (MON) in diets commonly fed to dairy (DAIRY; 50:50 forage to concentrate; four trials) and beef cattle (BEEF; 15:85 forage to concentrate; three trials) on rumen fermentation and CH4 production using a semi-continuous fermentation system. A 3 × 2 factorial arrangement was used and additives (0, 1.25, and 2.5% of EN; 0 and 4 mg/L of MON) were tested alone and combined (EN + MON) totaling six treatments. Rumen fluid was pooled from five nonadapted lactating cows fed 50:50 forage to concentrate diet 3 h after morning feeding, and 1 L of processed inoculum was transferred to 2.2-L vessels. Treatment diets were added to nylon bags which remained in the anaerobic fermentation of mixed rumen microorganisms for 48 h. Nitrate decreased CH4 production in DAIRY (24.7 vs. 32.1 mM/d; P < 0.01) and BEEF trials (33.5 vs. 43.5 mM/d; P < 0.01). Methane production was decreased by MON in DAIRY (26.3 vs. 32.1; P < 0.01) and BEEF (26.6 vs. 43.5 mM/d; P < 0.01). The combination of EN + MON further decreased CH4 in DAIRY (21.3 vs. 32.1 mM/d; P = 0.03) and BEEF (19.3 vs. 43.5 mM/d; P = 0.01). Nitrate did not affect major VFA production in DAIRY and BEEF trials, but significantly decreased digestion of protein (96.8 vs. 97.6%; P < 0.01) and starch (79.0 vs. 80.4%; P < 0.01) in DAIRY and NDF (29.3 vs. 32.5%; P < 0.01) and starch (88.5 vs. 90.3%; P < 0.01) in BEEF. Monensin significantly affected VFA pattern with an increase in propionate (P < 0.01) and a decrease on acetate (P < 0.01) production with consequent decrease on acetate-to-propionate ratio in DAIRY (1.6 vs. 2.0; P < 0.01) and BEEF (1.6 vs. 1.9; P < 0.01). Monensin decreased NDF digestion in BEEF only (29.3 vs. 32.5 %; P < 0.01). Significant concentrations of nitrate and nitrite were detected only for EN and EN + MON (P < 0.01). Nitrate and MON effectively decreased CH4 production when fed separately and the combination of additives additively decreased CH4 production.