A Study of Local Anaesthetics. Part 202. Determination of the Critical Micellar Concentration of CarbisocainiumChloride in Water Using Spectral Methods and the Probe Pyrene

Abstract

The micellization process of the local anaesthetic carbisocainium chloride in water was investigated by two spectral methods using the probe pyrene. First, the absorption spectroscopy in ultraviolet/Visible (UV/VIS) region was based on studying changes in characteristic absorption spectrum of pyrene in the presence of a surfactant. The resultant plot of the sum of absorbances for all the major pyrene peaks as a function of the total surfactant concentration shows, around the critical micellar concentration, a typical sigmoidal increase. Second, the fluorescence emission spectroscopy in UV/VIS region of spectrum by the probe pyrene was applied for the determination of the critical micellar concentration from the measurements of the pyrene I1/I3 ratio as a function of the surfactant concentration. The pyrene ratio data were fitted by the Boltzmann-type sigmoid of decreasing character. © 2013, Versita. All rights reserved.