Isolation and structural elucidation of a peptide derived from Edam cheese that inhibits β-lactoglobulin transport

Abstract

A peptide that inhibits β-lactoglobulin absorption in an in vitro Caco-2 cell model was isolated from Edam cheese. By 1H-NMR and N-terminal amino acid analyses, the active compound was identified as Asp-Lys-Ile-His-Pro-Phe. The sequence of the hexapeptide is the same as the amino acid sequence of residues 47-52 of β-casein. The hexapeptide shows remarkable inhibition of β-lactoglobulin transport at a concentration of 10-7 M. The possibility exists that this peptide can be applied practically to the prevention of milk-sensitive allergy.