The endophytic bacteria producing IAA (Indole Acetic Acid) in Arachis hypogaea

Abstract

Herlina L, Pukan KK, Mustikaningtyas D. 2017. The endophytic bacteria producing IAA (Indole Acetic Acid) in Arachis hypogaea. Cell Bio Dev 1: 31-35. Endophytic bacteria are bacteria living in plant tissue and forming colony without harms the host. Every cormophyte, plants that have a stem and root, may contain some endophytic bacteria which can produce biological compounds or secondary metabolites. The objective of the study was to obtain endophytic bacteria isolates from peanut plants (Arachis hypogaea) at three locations, and to test in vitro the ability of endophytic bacteria isolates for producing IAA, and also to analyze IAA on the growth and development of mungbean plants. The study was carried out in three stages; the first was the isolation of endophytic bacteria from the leaves, stems, and roots; the second was the test of in vitro endophytic bacteria isolates to know the ability of IAA production. IAA assay was measured by using a spectrophotometer with a wavelength of 535 nm, and the third was the introduction of IAA-producing endophytic bacteria in mungbean. The parameters observed were the length of sprouts and the number of lateral roots. The results showed that 16 isolates were selected based on IAA-producing ability. The isolates could produce different IAA with different morphological characteristics. After the fourth day of incubation, the highest and the lowest of IAA amount were 69.68 (mg L-1) and 8.50 (mg L-1) respectively. Isolates that produce high IAA levels are applied to mungbeans, it affects the number of lateral roots but it does not have effect on the length of the sprouts. DM and K1K1 isolates have the effect of increasing lateral root formation and are expected to be potential sources of bioactive metabolites.