Growth and Maintenance of Aedes albopictus Cell line, clone C6/36, in Diferrent Media

Abstract

The sensitivity of Aedes albopicts cell line, clone C6/36, to arbovirus isolation and growth has been being shown by several authors. The present work has verified which, among several media under the same conditions, would be the most efficient one for C6/36 cultivation and viral isolation. The L-15 medium has proved to be the best among others (MEM, DMEM and 199) with the quickest viral isolation (DEN-1). Also, in this medium, the samples could be observed until the 14th day, without significative cellular death. The results reccommend L-15 medium as the most efficíent and economic one for the purposes.