Production, purification, and identification of Glycine, N-(m-anisoyl)-methyl ester from Pseudomonas aeruginosa with antimicrobial and anticancer activities.

Abstract

The present research is a trial to extract antimicrobial and anticancer substance(s) from Pseudomonas aeruginosa. The bacterial isolate S1B20 exhibited the highest antimicrobial activity that was identified based on 16S rRNA gene sequence as Pseudomonas aeruginosa MG429777. Extraction of various antimicrobial substance(s) (both proteinous and non-proteinous substances) were carried out. Chloroform was established to be the strongest solvent for extraction of the antimicrobial substance, while the 60% ammonium sulfate saturation stage was the brightest to obtain active bacteriocin fraction. The molecular weight of the refined bacteriocins estimated as 124 kDa. Qualitative and quantitative results of GC-mass spectral analysis and IR spectroscopy of non-proteinous and proteinous antimicrobial agents were Oxime methoxyphenyl and Glycine, N-(m-anisoyl)-methyl ester. The research concerned the proteinous compound and proved to have a distinct antibacterial and antitumor force against three carcinoma cell lines.