Bacterial production and purification of phosphorylatable phosphoenolpyruvate carboxylase from tobacco

Abstract

Tobacco phosphoenolpyruvate carboxylase (PEPC) [EC 4.1.1.31] cDNA was efficiently expressed in E. coli under the control of the lacZ promoter. The enzyme, purified to homogeneity, had the same catalytic activity, and was phosphorylated in vitro by maize PEPC kinase. © 1996, Taylor & Francis Group, LLC. All rights reserved.