EFFICIENCY OF SILVER NANOPARTICLES (AgNPs) SYNTHESIS BASED ON SAMPLE PREPARATION OF MANGROVE BAITWORM (ANNELIDA, EUNICIDAE) MARPHYSA MORIBIDII IDRIS, HUTCHINGS AND ARSHAD, 2014

Abstract

Green synthesis of silver nanoparticles (AgNPs) is gaining interest due to its environmentally friendly, easy and biocompatible process. AgNPs synthesis using polychaete extract is relatively new but exhibits good potential. This study examines a local baitworm species, Marphysa moribidii, on its efficiency in synthesising AgNPs using two-sample preparations (fresh and frozen) with different weights. Samples were mixed with silver nitrate (AgNO3) before recording the colour changes. The ultraviolet-visible (UV-Vis) spectroscopy analysis, scanning electron microscope (SEM) and transmission electron microscope (TEM) were undertaken to detect the quantity and quality of synthesised AgNPs. Successful synthesis of AgNPs was observed for both samples as the solution colour changed from pinkish red to brown and confirmed with surface plasmon resonance peaks. Fresh extract with 5 g, 10 g and 15 g demonstrated higher reducing capability in AgNP synthesis than those frozen based on their peak uniformity in the UVVis spectrum. SEM and TEM images revealed spherical AgNP formation, which is the preferred shape for antimicrobial studies. X-ray diffraction and Fourier transform infrared spectroscopy found no trace of Ag contaminants from the environment. Further analysis and optimisation are required to reveal the reducing mechanism, especially using fresh samples, as it has a higher capability in synthesising AgNPs