The increasing use of zebrafish larvae for biomedical research applications is resulting in versatile models for a variety of human diseases. These models exploit the optical transparency of zebrafish larvae and the availability of a large genetic tool box. Here we present detailed protocols for the robotic injection of zebrafish embryos at very high accuracy with a speed of up to 2000 embryos per hour. These protocols are benchmarked for several applications: (1) the injection of DNA for obtaining transgenic animals, (2) the injection of antisense morpholinos that can be used for gene knock-down, (3) the injection of microbes for studying infectious disease, and (4) the injection of human cancer cells as a model for tumor progression. We show examples of how the injected embryos can be screened at high-throughput level using fluorescence analysis. Our methods open up new avenues for the use of zebrafish larvae for large compound screens in the search for new medicines. © 2013 The Authors.
Spaink, H. P., Cui, C., Wiweger, M. I., Jansen, H. J., Veneman, W. J., Marín-Juez, R., … Dirks, R. P. (2013). Robotic injection of zebrafish embryos for high-throughput screening in disease models. Methods, 62(3), 246–254. https://doi.org/10.1016/j.ymeth.2013.06.002