Aim: The aim of this study is to explore the function of miR-20a in osteosarcoma. Materials & methods: miR-20a expression was measured by real-time PCR. miR-20a mimics, inhibitor and scramble siRNA were transfected into osteosarcoma cells to observe effects on colony formation and tumor growth. Moreover, relationships of miR-20a with TAK1 were investigated by western blot and luciferase activity. Results: We found that miR-20a was downregulated in osteosarcoma, and overexpression of miR-20a reduced colony formation and tumor growth. Furthermore, the data revealed that the function of miR-20a was probably exerted via targeting the TAK1 expression. Overexpression of miR-20a sensitizes the osteosarcoma cells to chemotherapeutic drugs. Conclusion: Our data identify the role of miR-20a in osteosarcoma growth, indicating its potential application in chemotherapy.
Mendeley helps you to discover research relevant for your work.
CITATION STYLE
Yuan, G., Zhao, Y., Wu, D., Gao, C., & Jiao, Z. (2018). MiRNA-20a upregulates TAK1 and increases proliferation in osteosarcoma cells. Future Oncology, 14(5), 461–469. https://doi.org/10.2217/fon-2017-0490