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A simple, large-scale overexpression method of deriving carbon monoxide dehydrogenase II from thermophilic bacterium Carboxydothermus hydrogenoformans

Bioscience, Biotechnology and Biochemistry (2011) 75(7) 1392-1394
DOI: 10.1271/bbb.110159
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Abstract

We established an Na2S-free, large-scale overexpression system of deriving CODH II from thermophilic bacterium Carboxydothermus hydrogenoformans in Escherichia coli using a large-scale fermentor. Recombinant-CODH II showed a CO oxidation activity of 9,600 U/mg. In addition, recombinant-CODH II exhibited considerable CO2 reduction activity, of 16.9 U/mg.

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Inoue, T., Yoshida, T., Wada, K., Daifuku, T., Fukuyama, K., & Sako, Y. (2011). A simple, large-scale overexpression method of deriving carbon monoxide dehydrogenase II from thermophilic bacterium Carboxydothermus hydrogenoformans. Bioscience, Biotechnology and Biochemistry, 75(7), 1392–1394. https://doi.org/10.1271/bbb.110159

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