Reserch of the gene polymorphism TOX3/LOC643714 and the risk of breast cancer development in persons exposed to ionizing radiation after Chornobyl disaster

Abstract

Objective. The objective of this work was to identify and compare the polymorphism of the rs3803662 polymorphism of the TOX3/LOC643714 gene in breast cancer patients who have undergone ionizing radiation due to the Chornobyl accident and in patients without ionizing radiation (IR) in the history. Materials and methods. The determination of the rs3803662 polymorphism of the TOX3/LOC643714 gene was performed by polymerase chain reaction (PCR) in 83 patients with breast cancer: 42 subjects who were exposed to ionizing radiation due to the Chornobyl accident, 41 people without ionizing radiation in history and 17 controls in Ukraine without cancer pathology. In order to compare the obtained data on spontaneous and radiation-associated breast cancer and to calculate the differences in the frequencies of alleles and the risk of oncopathology, data from literature on control groups of the populations of the Russian Federation, Sweden, and the United Kingdom were used. Results. Comparing with the literature data and the group of exposed subjects, the homozygous carriers of the minor alleles of the TOX3/LOC643714 TT gene revealed an increased risk of developing breast cancer: OR = 2.89, p = 0.02 (CI 95% 1.17-7,16). In subjects without the influence of IR in history, the carrier of homozygous minor axis of the gene TOX3/LOC643714 TT is also associated with the risk of breast cancer: OR = 3.83, p = 0.0002 (CI 95% 0.82-14.14). In the homozygous carriers of the minor alleles of the TOX3/ LOC643714 gene exposed to IR, there was no increase in the risk of developing breast cancer (OR = 0.65, p = 0.46, CI 95% 0.21-2.04) compared with the control group of Ukrainian population. Conclusions. The carrier of homozygous minor alleles of the TOX3/LOC643714 gene is not a risk factor for the development of breast cancer under conditions of exposure to ionizing radiation in the study group of the Ukrainian population.