Replicatively senescent cells of Saccharomyces cerevisiae were obtained using the 'baby machine' method by immobilizing cells on CovaLink™ NH2 plates and allowing them to divide while exchanging medium and removing daughter cells. Centrifugation in a Percoll density gradient was employed for further purification of replicatively old yeast cells. Comparison of senescent cells showing more than 20 bud scars with cells from early stationary culture demonstrated a significant reduction of total and reduced glutathione and decrease of superoxide dismutase activity during replicative aging of yeast cells. © 2001 Federation of European Biochemical Societies.
Grzelak, A., Skierski, J., & Bartosz, G. (2001). Decreased antioxidant defense during replicative aging of the yeast Saccharomyces cerevisiae studied using the “baby machine” method. FEBS Letters, 492(1–2), 123–126. https://doi.org/10.1016/S0014-5793(01)02244-X