Recent advancements in molecular detection of Vibrio species in aquatic animals: A review

Abstract

Vibriosis is one of a major problem in aquaculture sector causes by Vibrio species and gives bad impact on economy and social development. In addition, number of the infected people with vibriosis is increasing every year through consuming of contaminated fi shes and other aquatic animals. To overcome this problem, researchers continuously develop new methods for identifi cation and detection of Vibrio species. Those methods can be categorized based on the principle applied; conventional culture method, antigen-antibody based assay, nucleic acid amplifi cation tech-nology and lateral fl ow dipstick. The conventional culture method is a basis in identifi cation of Vibrio but it is time consuming and requires skilled personnel. The nucleic acid amplifi cation technology such as isothermal amplifi cation assay in a combination of lateral fl ow dipstick is widely used since these assays offer rapid, easy to handle and high sensitivity and specifi city detection methods. This paper also reviews and describes the available application and limitations of the studies involving Vibrio detection methods of aquaculture fi eld. As a conclusion, the development of new technologies is very important to improve the detection of Vibrio species as well as increasing the number of food production to meet human demands.