Abundance of Yeast by Metagenomic Analysis of Eco-Enzymes Mangosteen peel

Abstract

Eco Enzyme has a million benefits because of its alcohol and acetic acid content, as well as various enzymes, so it has a role in killing and preventing pathogenic microorganisms, even treating wounds on the skin. Eco Enzyme has a pH of less than 4, so it is rich in organic acids and causes the death of pathogenic microorganisms. Eco Enzyme using mangosteen peel (Garcinia mangostana L.) is expected to enrich the content of yeast and enzymes that are beneficial for life. Mangosteen peel contains xanthone compounds that act as antioxidants and play a role in skin tissue regeneration and anti-aging. The yeast content in Eco Enzyme has yet to be wholly identified through microbial metagenomics to further analyze its benefits for life. Fifty milliliters of the Eco Enzyme mangosteen peel product was extracted using the Oxford Nanopore Technology kit. We calculated the concentration of extracted DNA using the NanoDrop spectrophotometer and Qubit fluorometer. Library preparation was carried out using the Kit from Oxford Nanopore Technology. DNA sequencing used nanopore sequencing and 16S DNA primers and operated with MinKNOW software version 23.04.5. DNA base calling using Guppy version 6.5.7, visualization using FASTQ and NanoPlot with NanoFilt filtering. The most abundant yeast species is Pichia kudriavzevii, found in Eco Enzyme mangosteen peel.