Determination of phenolic profile by HPLC-ESI-MS/MS and anti-inflammatory activity of crude hydroalcoholic extract and ethyl acetate fraction from leaves of Eugenia brasiliensis

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Abstract

Eugenia brasiliensis Lam., Myrtaceae, is used in folk medicine for anti-inflammatory diseases such asarthritis and rheumatism. This study investigated the anti-inflammatory activity and phenolic profile ofthe crude hydroalcoholic extract and ethyl acetate fraction from E. brasiliensis leaves. Crude hydroal-coholic extract and the ethyl acetate fraction were analyzed by HPLC-ESI-MS/MS in comparison tostandard phenolic compounds. The anti-inflammatory activity of the crude hydroalcoholic extract (1,10 and 25 mg kg−1) and the ethyl acetate fraction (10, 25 and 50 mg kg−1) was evaluated in a swissmouse model of acute pleurisy induced by carrageenan, being the total cell count, exudation and analysisof nitrite/nitrate the inflammation parameters. HPLC-ESI-MS/MS analysis revealed apigenin, catechin,galangin, isoquercetin, myricetin, quercetin and rutin. Crude hydroalcoholic extract and ethyl acetatefraction were effective in inhibiting cell migration in all tested doses. Crude hydroalcoholic extract waseffective in inhibiting exudation only at the 10 mg kg−1dose; ethyl acetate fraction was effective in alltested doses. Results for nitrite/nitrate levels reveals that only the ethyl acetate fraction was effectiveat the tested doses. This is the first report of the presence of isoquercetin, galangin and apigenin in thisspecies. Results from the phytochemical analysis enhance the chemical knowledge of this species. In the future, together with more studies, validation of its popular use in inflammatory diseases is possible.

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Siebert, D. A., Bastos, J., Spudeit, D. A., Micke, G. A., & Alberton, M. D. (2017). Determination of phenolic profile by HPLC-ESI-MS/MS and anti-inflammatory activity of crude hydroalcoholic extract and ethyl acetate fraction from leaves of Eugenia brasiliensis. Brazilian Journal of Pharmacognosy, 27(4), 459–465. https://doi.org/10.1016/j.bjp.2017.01.008

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