The development of a cell array and its combination with laser-scanning cytometry allows a high-throughput analysis of nuclear DNA content

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Abstract

We developed a cell array device for analyzing cellular characteristics such as DNA ploidy, numerical chromosomal aberrations, and antigen expression in multiple specimens in a single experiment. Fifty (10× 5) spots, 2 mm in diameter, were arrayed in an area of 30× 16 mm on a glass slide, and ∼1,000 cells were placed on each spot. To demonstrate the usefulness of the cell array, we measured nuclear DNA content using laser-scanning cytometry for DNA ploidy analysis in nine human tumor cell lines and normal lymphocytes. Combining the cell array with laser-scanning cytometry allows not only measurement of nuclear DNA content for 50 samples but also easy comparison of DNA ploidy among the samples in a single experiment. In addition, we used the cell array for fluorescence in situ hybridization using a DNA probe specific for the pericentromeric region of chromosome 11. © 2000 American Society for Investigative Pathology.

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Oode, K., Furuya, T., Harada, K., Kawauchi, S., Yamamoto, K., Hirano, T., & Sasaki, K. (2000). The development of a cell array and its combination with laser-scanning cytometry allows a high-throughput analysis of nuclear DNA content. American Journal of Pathology, 157(3), 723–728. https://doi.org/10.1016/S0002-9440(10)64585-3

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