Single molecule studies of RNA polymerase II transcription in vitro

Abstract

Eukaryotic mRNA transcription by RNA polymerase II (RNAP II) is the first step in gene expression and a key determinant of cellular regulation. Eelucidating the mechanism by which RNAP synthesizes RNA is therefore vital to determining how genes are controlled under diverse biological conditions. Significant advances in understanding RNAP transcription have been achieved using classical biochemical and structural techniques; however, aspects of the transcription mechanism cannot be assessed using these approaches. The application of single-molecule techniques to study RNAP IIii transcription has provided new insight only obtainable by studying molecules in this complex system one at a time. © 2014 Landes Bioscience.