Studies on the Optimal Immunization Schedule of Experimental Animals: VI: Antigen Dose-Response of Aluminum Hydroxide-Aided Immunization and Booster Effect under Low Antigen Dose

Abstract

The dose-response relationships of a viomycin (VM) immunogen for total immunoglobulin (Ig) G and anti-VM antibody response of mouse using aluminum hydroxide as adjuvant was studied. The condition required to absorb a protein on aluminum gel was first established. The effective immunogen dose for total and specific IgG response of mouse using aluminum hydroxide as the adjuvant was found to be in the narrow range of 5 to 20 jig and 10 pg per mouse was optimal. The most effective number and intervals of booster injections were studied: when mice were immunized with a lower antigen dose than the optimal, both the number and interval period of booster injections greatly affected the immune response; the more boosters were given, the higher was the response level of specific IgG. The results are contrary to those obtained by immunizing with the optimal or a higher antigen dose. © 1990, The Pharmaceutical Society of Japan. All rights reserved.