A simple fabricated microfluidic chip for urine sample-based bladder cancer detection

Abstract

Bladder cancer is the most common malignancy of the urinary system with high morbidity and worldwide mortality. However, there is no clinically noninvasive method for high sensitivity, specificity and low-cost detection. A new microfluidic chip is designed for the detection of bladder cancer in a urine sample in the current research. Polydimethylsiloxane (PDMS) is used to form a channel using a cast molding method, with a sliding glass coverslip as the substrate. When the inner surface of the chip is modified by silanization to fix a specific antibody, urine samples and standard protein solutions are pumped into the chip to detect bladder cancer cells and biomarkers. The results show that the chip can quantitatively detect the difference in bladder cancer cells and biomarkers between patients' and volunteers' urine samples. The sensitivity and the specificity of the microfluidic chip are 87.1% and 100%, respectively. This study successfully establishes a novel microfluidic chip for the detection and diagnosis of bladder cancer, providing new ideas and a powerful platform for the detection and diagnosis of bladder cancer.