Diagnosis and quantitative detection of HSV DNA in samples from patients with suspected herpes simplex encephalitis

17Citations
Citations of this article
19Readers
Mendeley users who have this article in their library.

Abstract

Diagnosis of herpes simplex encephalitis (HSE) is based on the detection of herpes simplex virus (HSV) DNA in patients' CSF samples. HSV DNA quantitation has the potential for estimating the efects of antiviral therapy. The aim of this study was to diagnose HSV DNA in HSE suspected patients and the quantitative analysis of its genome using real-time PCR to assess the value of the viral load in the course of antiviral treatment. The CSF samples were collected from 236 consecutive HSE suspected patients from November 2004 to May 2008. Upon DNA extraction, the samples were analyzed by Real-Time PCR assay. A set of primers amplifed a common sequence of HSV glycoprotein B gene. The copy numbers of unknown samples were expressed via a standard curve drawn with a known amount of amplifed cloned plasmid. Of the 236 samples, 137 (58%) came from males and 99 (42%) from females. The HSV genome was detected in 22 (9.3%) patients by PCR, 13 males/9 females. Serial CSF samples were available from 10 of the 22 patients. The range of the HSV DNA copy numbers in the clinical samples ranged from 2.5 × 102 to 1.7 × 106 copies/mL of CSF. Quantitative PCR results can be helpful in evaluating the eficacy of antiviral therapy in the above-mentioned patients. There is an association between the initial viral load and the duration of treatment course. © Elsevier Editora Ltda.

Cite

CITATION STYLE

APA

Ziyaeyan, M., Alborzi, A., Haghighi, A. B., Jamalidoust, M., Moeini, M., & Pourabbas, B. (2011). Diagnosis and quantitative detection of HSV DNA in samples from patients with suspected herpes simplex encephalitis. Brazilian Journal of Infectious Diseases, 15(3), 211–214. https://doi.org/10.1016/S1413-8670(11)70177-5

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free