Directed evolution has emerged in just a few years as one of the most effective approaches to adapting bio- catalysts to the performance require- ments of industrial and medical appli- cations. Directed evolution mimics the processes of Darwinian evolution in a test tube, combining random mutagenesis and/or recombination with screening or selection for enzyme variants that have the desired properties1. A recent conference* that convened practitioners from industry and academia surveyed current efforts and highlighted the rapid progress the field has enjoyed. In addition to impressive demonstrations of enzyme engineering, there were a variety of challenging problems and approaches to laboratory evolution.
Schmidt-Dannert, C., & Arnold, F. H. (1999). Directed evolution of industrial enzymes. In Trends in Biotechnology (Vol. 17, pp. 135–136). https://doi.org/10.1016/S0167-7799(98)01283-9