Discovery and validation of an inflammatory protein-driven gastric cancer signature (INPROGAS) using antibody microarray-based oncoproteomics

  • M. P
  • A. C
  • E. C
  • et al.
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Abstract

This study aimed to improve gastric cancer (GC) diagnosis by identifying and validating an INflammatory PROtein-driven GAstric cancer Signature (hereafter INPROGAS) using low-cost affinity proteomics. The detection of 120 cytokines, 43 angiogenic factors, 41 growth factors, 40 inflammatory factors and 10 metalloproteinases was performed using commercially available human antibody microarray-based arrays. We identified 21 inflammation-related proteins (INPROGAS) with significant differences in expression between GC tissues and normal gastric mucosa in a discovery cohort of matched pairs (n=10) of tumor/normal gastric tissues. Ingenuity pathway analysis confirmed the "inflammatory response", "cellular movement" and "immune cell trafficking" as the most overrepresented biofunctions within INPROGAS. Using an expanded independent validation cohort (n = 22), INPROGAS classified gastric samples as "GC" or "non-GC" with a sensitivity of 82% (95% CI 59-94) and a specificity of 73% (95% CI 49-89). The positive predictive value and negative predictive value in this validation cohort were 75% (95% CI 53-90) and 80% (95% CI 56-94), respectively. The positive predictive value and negative predictive value in this validation cohort were 75% (95% CI 53-90) and 80% (95% CI 56-94), respectively. Antibody microarray analyses of the GC-associated inflammatory proteome identified a 21-protein INPROGAS that accurately discriminated GC from noncancerous gastric mucosa.

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APA

M., P.-C., A., C.-C., E., C.-P., C., O.-F., S., C., S., F., … J., J. (2014). Discovery and validation of an inflammatory protein-driven gastric cancer signature (INPROGAS) using antibody microarray-based oncoproteomics. Oncotarget. J.A. Menendez, Girona Biomedical Research Institute(IDIBGI), Girona, Catalonia, Spain. E-mail: jmenendez@iconcologia.net: Impact Journals LLC. Retrieved from http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=download&path%5B%5D=1879&path%5B%5D=2475

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