Functional activity of N- and C-terminal fluorescent fusion proteins between STAT6 and EGFP was demonstrated through IL-4-dependent transcriptional activation and nuclear translocation. The N-terminal (EGFP-STAT6) fusion protein appeared to be more active than the C-terminal fusion. In HEK-293 cells both fusion proteins formed fluorescent nuclear foci following IL-4 stimulation, but in HeLa cells nuclear accumulation was homogeneous. Stimulation of the NF-κB pathway through TNFα treatment, or expression of p65-EGFP fusion protein, repressed both basal STAT6-dependent transcriptional activity and the extent of activation in response to IL-4. This indicates a novel mechanism of inhibition of STAT6 signalling by NF-κB activation. © 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
Nelson, G., Wilde, G. J. C., Spiller, D. G., Sullivan, E., Unitt, J. F., & White, M. R. H. (2002). Dynamic analysis of STAT6 signalling in living cells. FEBS Letters, 532(1–2), 188–192. https://doi.org/10.1016/S0014-5793(02)03672-4