Dynamic regulation of uncoupling protein 2 content in INS-1E insulinoma cells

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Abstract

Uncoupling protein 2 (UCP2) regulates glucose-stimulated insulin secretion in pancreatic beta-cells. UCP2 content, measured by calibrated immunoblot in INS-1E insulinoma cells (a pancreatic beta-cell model) grown in RPMI medium, and INS-1E mitochondria, was 2.0 ng/million cells (7.9 ng/mg mitochondrial protein). UCP2 content was lower in cells incubated without glutamine and higher in cells incubated with 20 mM glucose, and varied from 1.0-4.4 ng/million cells (2.7-14.5 ng/mg mitochondrial protein). This dynamic response to nutrients was achieved by varied expression rates against a background of a very short UCP2 protein half-life of about 1 h. © 2008 Elsevier B.V. All rights reserved.

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Azzu, V., Affourtit, C., Breen, E. P., Parker, N., & Brand, M. D. (2008). Dynamic regulation of uncoupling protein 2 content in INS-1E insulinoma cells. Biochimica et Biophysica Acta - Bioenergetics, 1777(10), 1378–1383. https://doi.org/10.1016/j.bbabio.2008.07.001

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