Natural contamination of manitoba barley by 3,15-diacetyldeoxynivalenol and its detection by immunochromatography

Abstract

Contamination of Canadian barley samples by 3,15-diacetyldeoxynivalenol was detected by enzyme immunoassays combined with liquid chromatography and was confirmed by gas chromatography-mass spectrometry. This is the first reported natural occurrence of this mycotoxin. The barley was infected mainly with Fusarium graminearum. Deoxynivalenol, 3-acetyldeoxynivalenol, 15- acetyldeoxynivalenol, and zearalenone were also found.