Virus Elimination by Meristem-Tip Culture

Abstract

Meristem-tip culture is one ofthe most widely used methods for virus elimination from infected plants and production ofvirus-free plants. Apical meristem culture is a proven means ofclonal propagation and also for eliminating viruses from infected plants. It exhibits inherent genetic stability and tremendous growth potential, making it useful material for long-term storage of virus-free germplasm. Also, due to the small length of the meristem explant, this technique will have the potential for eliminating other pathogenic organisms in addition to viruses. Meristem culture is more effective in eliminating viruses when it is combined with thermotherapy and chemotherapy. Pre-treatment of infected plants with higher tempera- ture and usage of antiviral agents in the meristem regeneration medium have been reported to be an effective