Les jardins partagés franciliens, scènes de participation citoyenne ?

Abstract

Advances in sequencing technologies have made the investigation of microbial ecology and community dynamics more tractable. The critical fi rst step in such analyses is the effi cient and representative recovery of PCR-competent DNA from complex environmental samples. All extraction protocols contain inherent biases, meaning that choice of method involves compromise between various factors, including effi ciency, yield, universality, and representative extraction. Here, details are given for a routine method used in our laboratory to extract DNA from soils, sediments, biofilms, roots, and fungi.