Stoichiometry of natural bacterial assemblages from lakes located across an elevational gradient

Abstract

Heterotrophic bacteria are thought to be phosphorus-rich organisms with relatively homeostatic stoichiometry, but the elemental composition of natural bacterial communities has rarely been assessed. Here we tested whether bacterial stoichiometry changes with the trophic status of lakes by assessing the elemental composition of the bacterial-dominated (hereafter microbial) fraction together with that of the dissolved and seston fractions in 11 lakes situated along an elevational gradient. The stoichiometry of these three size-fractions was analyzed during the thermal stratification and mixing periods in composite water samples and in the water layer of the deep chlorophyll-a maximum. In addition, we analyzed the relative abundance of the most common bacterial groups in the lakes. Our results show that the microbial fraction was always enriched in phosphorus compared to the dissolved fraction, irrespectively of the lake trophic status. Further, they indicate that the elemental composition of bacteria in mountain lakes is at least seasonally very dynamic, resulting not only from changes in the nutrient ratios of the resource itself, but probably from changes in the composition of the dominant bacterial taxa too, though at the taxonomic level analyzed, we did not find evidence for this.