PSD-95 mediates membrane clustering of the human plasma membrane Ca2+ pump isoform 4b

20Citations
Citations of this article
15Readers
Mendeley users who have this article in their library.

Abstract

Besides the control of global calcium changes, specific plasma membrane calcium ATPase (PMCA) isoforms are involved in the regulation of local calcium signals. Although local calcium signaling requires the confinement of signaling molecules into microdomains, little is known about the specific organization of PMCA molecules within the plasma membrane. Here we show that co-expression with the postsynaptic density-95 (PSD-95) scaffolding protein increased the plasma membrane expression of PMCA4b and redistributed the pump into clusters. The clustering of PMCA4b was fully dependent on the presence of its PDZ-binding sequence. Using the fluorescence recovery after photobleaching (FRAP) technique, we show that the lateral membrane mobility of the clustered PMCA4b is significantly lower than that of the non-clustered molecules. Disruption of the actin-based cytoskeleton by cytochalasin D resulted in increased cluster size. Our results suggest that PSD-95 promotes the formation of high-density PMCA4b microdomains in the plasma membrane and that the membrane cytoskeleton plays an important role in the regulation of this process. © 2008 Elsevier B.V.

Cite

CITATION STYLE

APA

Padányi, R., Pászty, K., Strehler, E. E., & Enyedi, Á. (2009). PSD-95 mediates membrane clustering of the human plasma membrane Ca2+ pump isoform 4b. Biochimica et Biophysica Acta - Molecular Cell Research, 1793(6), 1023–1032. https://doi.org/10.1016/j.bbamcr.2008.11.007

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free