Bioaktivitas Ekstrak Turbinaria decurrens Sebagai Antitumor (Hela Dan T47d) Serta Efeknya Terhadap Proliferasi Limfosit

  • Fajarningsih N
  • Nursid M
  • Wikanta T
  • et al.
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Abstract

ABSTRAK Riset mengenai bioaktivitas ekstrak makroalga Turbinaria decurrens terhadap proliferasi sel limfosit dan sitotoksisitas terhadap sel lestari tumor HeLa dan T47D telah dilakukan. Uji toksisitas dilakukan dengan metode Brine Shrimp Lethality Test (BSLT), sedangkan uji sitotoksisitas terhadap sel tumor HeLa dan T47D dilakukan dengan metode 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide]) assay (metode MTT), uji proliferasi limfosit juga dilakukan dengan metode MTT. Hasil uji BSLT menunjukkan bahwa fraksi n-heksana T. decurrens memiliki toksisitas tertinggi dengan LC 50 sebesar 672,59 ppm sehingga tergolong toksik. Sementara itu, ekstrak T. decurrens mempunyai aktivitas sitotoksisitas terhadap sel tumor HeLa (LC 50 ekstrak kasar = 29,9 ppm), namun tidak efektif terhadap sel T47D (LC 50 ekstrak kasar >1.000 ppm). Fraksi n-heksana T. decurrens merupakan fraksi yang menunjukkan aktivitas sitotoksisitas terbaik terhadap sel tumor HeLa dengan LC 50 sebesar 15,1 ppm. Ekstrak kasar, fraksi metanol, fraksi etil asetat, dan fraksi n-heksana dari makroalga T. decurrens mampu meningkatkan proliferasi sel limfosit manusia. Metabolit sekunder dari fraksi n-heksana T. decurrens sangat prospektif dikembangkan sebagai senyawa antitumor. ABSTRACT: Bioactivity of Turbinaria decurrens extract as an antitumor (HeLa and T47D) and its effects on lymphocyte proliferation. By: Nurrahmi Dewi Fajarningsih, Muhammad Nursid, Thamrin Wikanta, and Endar Marraskuranto Studies to determine the bioactivity of macroalga Turbinaria decurrens extract againts HeLa and T47D tumors and its effect on human lymphocite proliferation have been carried out. Toxicity assay was conducted using Brine Shrimp Lethality Test (BSLT) method while cytotoxicity assay against HeLa, T47D cell lines and for lymphocite proliferation were carried out using 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide]) assay (MTT method). The results of BSLT test showed that T. decurrens n-hexane fraction has the highest toxicity with Lethal Concentration 50 (LC 50) value of 672.59 ppm. T. decurrens crude ethanol extract showed a high antitumor activity against HeLa cell lines with LC 50 value of 29.9 ppm. On other hand, the extract showed low bioactivity against T47D cell lines (LC 50 value >1,000 ppm). N-hexane fraction of T. decurrens showed the best bioactivity against HeLa cell lines with LC 50 value of 15.1 ppm, whereas crude ethanol extract, methanol fraction, ethyl acetate fraction, and n-hexane fraction of T. decurrens increased the human lymphocyte proliferation. There were evidences that secondary metabolites of T. decurrens n-hexane fraction had a good potential for the development of nutraceutical products.

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APA

Fajarningsih, N. D., Nursid, M., Wikanta, T., & Marraskuranto, E. (2008). Bioaktivitas Ekstrak Turbinaria decurrens Sebagai Antitumor (Hela Dan T47d) Serta Efeknya Terhadap Proliferasi Limfosit. Jurnal Pascapanen Dan Bioteknologi Kelautan Dan Perikanan, 3(1), 21. https://doi.org/10.15578/jpbkp.v3i1.6

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