Agonistically acting autoantibodies directed against the adrenergic beta-1 receptor (beta1-AABs) are a pathogenic factor in diseases of the heart and circulatory system such as dilated cardiomyopathy. Here we describe the detection of such functionally active beta1-AABs from serum samples using spontaneously beating neonatal rat cardiomyocytes, which express the fully functional adrenergic beta-1 receptor coupled with the signal transduction pathway that induces chronotropy. With serum samples added (containing beta1-AABs), an increased positive chronotropic effect is caused that can be blocked by the subsequent addition of specific beta-blockers (abolishing this chronotropic response). The return to the basal beat rate of the cells by the addition of a beta-blocker proves the adrenergic beta-1 receptor specificity of the serum sample.
CITATION STYLE
Davideit, H., Haberland, A., Bartel, S., Schulze-Rothe, S., Müller, J., & Wenzel, K. (2019). Determination of agonistically acting autoantibodies to the adrenergic beta-1 receptor by cellular bioassay. In Methods in Molecular Biology (Vol. 1901, pp. 95–102). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8949-2_8
Mendeley helps you to discover research relevant for your work.