Potential of dynamic bacterial communities in the bio-corrosion process: A proof study with surface morphology of metal coupons

Abstract

Bio-corrosion is a well-known phenomenon of corrosion caused by bacterial communities. It is considered as a worldwide problem as it causes billion-dollar damages to the pipeline industries (mainly oil and gas) each year. Therefore, this investigation was undertaken to understand the significance of bacterial communities in the bio-corrosion system by studying the physical alteration in the metal surface of coupons through different techniques (EIS, XRD, FT-IR and SEM) and the community identification of consortia responsible for the corrosion. Furthermore, supporting data were obtained from APS reductase assays and DAPI microscopy. The EIS plots suggested that the metal coupons in a biotic system were more prone to corrosion than the coupons in an abiotic system. FT-IR analysis of the biotic system validated the presence of magnetite (Fe3O4), goethite (α-FeOOH) and lepidocrocite (γ-FeOOH); the XRD spectrum confirmed the presence of oxide and sulphide of iron (Fe3O4 and FeS), which are considered as notable compounds for corroding substances. The community profile indicated the presence of mixed anaerobic consortia containing Firmicutes and Proteobacteria (beta and delta) in the cultured sample. The presence of Desulfovibro sp. and Clostridium sp. in the consortium revealed a synergistic effect, where the by-product of one species acted as a carbon source for the other species, which further established the bio-corrosion process by depositing oxides of iron and sulphur on the metal coupon surface. This study signifies that a mixed culture has a greater impact on the bio-corrosion process than the pure and single culture of Desulfovibro sp. Furthermore, this study also provides a bio-monitoring strategy for the pipeline industries.