Antioxidant and antibacterial activities of methanol, ethanol and aqueous extracts of leaf and root of Gentiana kurroo Royle

Abstract

Phytochemical content, antioxidant and antimicrobial activity in various extracts, i.e., methanol, ethanol and water (aqueous), prepared from leaves and roots of Gentiana kurroo Royle were evaluated. Highest phenol, flavonoid, anthocyanin and gallotanin content was observed in methanolic root extract, i.e., 77.85 +/- 3.91, 44.761 +/- 0.63, 6.253 +/- 0.14 and 3.956 +/- 0.09 mg/g extract, respectively. Aqueous extract showed least phytochemical content among all the extracts. Highest antioxidant activity was also observed in methanol root and leaf extracts. Highest DPPH and superoxide radical scavenging activity (IC50; mu g/ml), i.e., 316.290 +/- 1.72 and 142.759 +/- 0.258, respectively was observed in methanolic root extract. The extract also showed highest metal ion chelating ability (EC50), i.e., 1162.525 +/- 81.293, mg/ml. There was a positive correlation between phytochemical and antioxidant activities in different extracts. The extracts showed significant (p