Continuity of orientation columns between superficial and deep laminae of the cat primary visual cortex.

Abstract

1. Recent reports of a marked and consistent dislocation between orientation columns in the superficial and deep layers of cat striate cortex (Bauer, 1982, 1983) directly contradict the traditional view of the system (Hubel & Wiesel, 1962). This has considerable implications for our current understanding of cortical organization, and in order to clarify the issue we have carried out experiments to test the continuity of the columnar system with depth, in central regions of area 17. 2. In twenty‐four penetrations, eighteen of which were placed as perpendicular as possible to the surface of the cortex, orientation preference was assessed at regular intervals both qualitatively and using a randomly interleaved quantitative technique. The distribution of preferred orientations was analysed with reference to a detailed histological reconstruction of the electrode track, including the location of laminar boundaries and the course of radial tiers of cells and capillaries. 3. From a further series of eighteen near‐perpendicular penetrations, the change in average orientation between one superficial and one deep layer recording site was compared with the deviation of the track from perpendicular to the surface and hence parallel to the orientation columns. 4. In penetrations perpendicular to the surface of the cortex, orientation preference showed little variation between superficial and deep laminae. In oblique penetrations, preferred orientation generally changed according to a single, smooth trend. Those irregularities that were encountered were confined to oblique penetrations, and were distributed throughout the cortical laminae. 5. In conclusion, our evidence does not support the presence of a systematic discontinuity with depth within the orientation columnar system. It is therefore entirely consistent with earlier evidence on the subject. © 1986 The Physiological Society