Biosensor based on TiO2 Nanofibers Prepared Using High Energy Electron Beam Treatment for Rapid and Efficient Glucose Determination

Abstract

TiO2 nanofibers (p-TDNF) were synthesized by electrospinning and highly active TiO2 nanofibers (m-TDNF) were obtained by electron beam treatment of p-TDNF and assessed for the enzyme immobilization and biosensing applications. Studies have shown surface defects and Ti3+ are formed on m-TDNF. Significantly, the better electron conduction properties were exhibited for glucose oxidase (GOD) immobilized on m-TDNF, and favorable electrocatalytic properties for glucose detection can be acquired. Impressively, GOD/m-TDNF/Nafion/GCE glucose biosensor exhibits high sensitivity of 12.5 µA·mM−1cm−2 and low detection limit of 0.9 µM as well as excellent selectivity and stability. Moreover, the accurate glucose determination and good recoveries in human blood serum implied their enormous potentiality in the practical applications. These results indicate that the preparation of m-TDNF offers a novel idea for the development of glucose biosensors.