A protocol for extraction and purification of high-quality and quantity bacterial DNA applicable for genome sequencing: a modified version of the Marmur procedure.

Abstract

Here we present a modified version of the Marmur procedure (Marmur, 1961) for extraction, isolation and purification of bacterial DNA. The protocol is effective in providing large amounts of high quality and high molecular weight genomic DNA from Gram-negative and Gram-positive bacteria, suitable for numerous downstream applications, including next-generation sequencing.