Optimization of laccase production from a newly isolated Trametes sp. EDN134

Abstract

Laccase is one important enzyme in decolorization of textile dyes. This research aims to study the optimization of laccase production from Trametes sp. EDN134 under several variation of substrates (bagasse, bamboo, Jabon wood, oil palm empty fruit bunch (OPEFB), and sorghum fibers), time incubation (1-15 d), growth temperature (25, 27, 30, 32, 35 and 37oC), and concentrations of inducer CuSO4 (1, 2, 3, 4 and 5 mM). The results showed that optimum laccase specific activity (14,838 U/g) detected in the OPEFB medium at 10 days incubation, temperature 30oC and CuSO4 2 mM. The study suggests that these parameters could be used for the optimization of laccase production from a newly isolated Trametes sp. EDN134.