Sputum and bronchial submucosal IL-13 expression in asthma and eosinophilic bronchitis

127Citations
Citations of this article
36Readers
Mendeley users who have this article in their library.

Abstract

Nonasthmatic eosinophilic bronchitis is a condition characterized by the presence of eosinophilic airway inflammation in the absence of airflow obstruction or airway hyperresponsiveness. In asthma, the T H2-type cytokine IL-13 has been implicated in the development of airway inflammation and hyperresponsiveness. Whether the expression of IL-13 is different between these 2 conditions is unknown. We sought to investigate whether IL-13 expression is increased in asthma compared with eosinophilic bronchitis. Sputum samples from subjects with mild asthma (n = 30) and eosinophilic bronchitis (n = 15) and normal controls (n = 16) were dialyzed, and IL-13 concentration was measured by ELISA. In a subgroup of these patients, IL-13 protein expression in bronchial biopsies was assessed by immunohistochemistry. The concentration of sputum IL-13 was higher in patients with mild asthma than in normal controls (P =. 03) and in patients with eosinophilic bronchitis (P =. 03). The median (interquartile range) number of IL-13 + cells/mm 2 submucosa was significantly higher in asthma 4 (8) than eosinophilic bronchitis 1.7 (1.9) and normal controls 0.5 (1.1; P =. 004). Eighty-three percent of the cells expressing IL-13 in the submucosa were eosinophils, and 8% were mast cells. The median (interquartile range) proportion of eosinophils that expressed IL-13 was higher in the subjects with asthma, 16 (10)%, than those with eosinophilic bronchitis, 7 (3)% (P =. 02). The increased expression of IL-13 in asthma compared with eosinophilic bronchitis supports the concept that IL-13 may play a critical role in the pathophysiology of asthma.

Cite

CITATION STYLE

APA

Berry, M. A., Parker, D., Neale, N., Woodman, L., Morgan, A., Monk, P., … Brightling, C. E. (2004). Sputum and bronchial submucosal IL-13 expression in asthma and eosinophilic bronchitis. Journal of Allergy and Clinical Immunology, 114(5), 1106–1109. https://doi.org/10.1016/j.jaci.2004.08.032

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free