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Efficient screening of environmental isolates for Saccharomyces cerevisiae strains that are suitable for brewing

Bioscience, Biotechnology and Biochemistry (2014) 78(6) 1086-1089
DOI: 10.1080/09168451.2014.910098
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Abstract

We developed an efficient screening method for Saccharomyces cerevisiae strains from environmental isolates. MultiPlex PCR was performed targeting four brewing S. cerevisiae genes (SSU1, AWA1, BIO6, and FLO1). At least three genes among the four were amplified from all S. cerevisiae strains. The use of this method allowed us to successfully obtain S. cerevisiae strains.

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Fujihara, H., Hino, M., Takashita, H., Kajiwara, Y., Okamoto, K., & Furukawa, K. (2014). Efficient screening of environmental isolates for Saccharomyces cerevisiae strains that are suitable for brewing. Bioscience, Biotechnology and Biochemistry, 78(6), 1086–1089. https://doi.org/10.1080/09168451.2014.910098

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