A compact and lightweight liquid chromatography system is presented with overall dimensions of 26 cm width × 18 cm length × 21 cm height and weight of 2 kg. This system comprises a battery-operated compact electroosmotic pump, a manual injector, a microfluidic chip device containing a packed column and an electrochemical detector, and a USB buspowered potentiostat. The pumping system was designed for microfluidic-based reversed-phase liquid chromatography in which an electroosmotically generated water stream pushes the mobile phase via a diaphragm for the output. The flow rate ranged from 0 to 10 μL/min and had a high degree of precision. The pumping system operated continuously for over 24 h with dry batteries. The column formed in the microfluidic device was packed with 3-μm ODS particles with a length of 30 mm and a diameter of 0.8 mm. The results presented herein demonstrate the performance of the pumping system and the column using alkylphenols, catecholamine, catechin, and amino acids.
CITATION STYLE
Ishida, A., Fujii, M., Fujimoto, T., Sasaki, S., Yanagisawa, I., Tani, H., & Tokeshi, M. (2015). A portable liquid chromatograph with a battery-operated compact electroosmotic pump and a microfluidic chip device with a reversed phase packed column. Analytical Sciences, 31(11), 1163–1169. https://doi.org/10.2116/analsci.31.1163
Mendeley helps you to discover research relevant for your work.