A portable liquid chromatograph with a battery-operated compact electroosmotic pump and a microfluidic chip device with a reversed phase packed column

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Abstract

A compact and lightweight liquid chromatography system is presented with overall dimensions of 26 cm width × 18 cm length × 21 cm height and weight of 2 kg. This system comprises a battery-operated compact electroosmotic pump, a manual injector, a microfluidic chip device containing a packed column and an electrochemical detector, and a USB buspowered potentiostat. The pumping system was designed for microfluidic-based reversed-phase liquid chromatography in which an electroosmotically generated water stream pushes the mobile phase via a diaphragm for the output. The flow rate ranged from 0 to 10 μL/min and had a high degree of precision. The pumping system operated continuously for over 24 h with dry batteries. The column formed in the microfluidic device was packed with 3-μm ODS particles with a length of 30 mm and a diameter of 0.8 mm. The results presented herein demonstrate the performance of the pumping system and the column using alkylphenols, catecholamine, catechin, and amino acids.

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Ishida, A., Fujii, M., Fujimoto, T., Sasaki, S., Yanagisawa, I., Tani, H., & Tokeshi, M. (2015). A portable liquid chromatograph with a battery-operated compact electroosmotic pump and a microfluidic chip device with a reversed phase packed column. Analytical Sciences, 31(11), 1163–1169. https://doi.org/10.2116/analsci.31.1163

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